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Image analysis and definition of nuclear phenotypes*

 

 

Benedicto de Campos Vidal

Departamento de Biologia Celular, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, Brasil.

 

 

ABSTRACT

Some basic topics concerned with the extraction of textural and geometric information from cell nucleus images as well as description and characterization of chromatin supraorganization and consequent classification of nuclear phenotypes are presented.

Keywords: Image analysis; nuclear phenotypes.

 

 

REFERENCES

Altman, P.L. and Katz, D.D. (1976). Cell Biology. Federation of American Societies for Experimental Biology. Bethesta, Maryland, pp. 371.

Bucher, O. and Horisberg, B. (1950). Karyometrische Untersuchungen an Gewebekulturen. Acta Anat. 9: 258-278.

Einstein, A.J., Barba, J., Unger, P.D. and Gil, J. (1994). Nuclear diffuseness as a measure of texture: definition and application to the computer-assisted diagnosis of parathyroid adenoma and carcinoma. J. Microsc. 176: 158-166.

Haralick, R.M., Shanmugam, K. and Dinstein, I. (1973). Textural features for image classification. IEEE Trans. Syst. Man & Cybern. 3: 610-621.

Mello, M.L.S. (1989). Nuclear fusion and change in chromatin packing state in response to starvation in Triatoma infestans. Rev. Bras. Genét. 12: 485-498.

Mello, M.L.S. and Chambers, A.F. (1993). Image analysis of Feulgen-stained transformed NIH 3T3 cells differing in p21 expression and ras-induced metastatic ability. Anal. Quant. Cytol. Histol. 16: 113-123.

Mello, M.L.S. and Russo, J. (1990). Image analysis of Feulgen-stained c-H-ras-transformed NIH/3T3 cells. Biochem. Cell Biol. 68: 1026-1031.

Mello, M.L.S., Vidal, B.C., Golembieski, W. and Russo, J. (1992). Image analysis of NIH/3T3 cells transformed with DNA of 4-nitroquinoline 1-oxide treated human breast epithelial cells. Biochem. Cell Biol. 70: 109-116.

Mello, M.L.S., Vidal, B.C., Planding, W. and Schenck, U. (1994). Image analysis: video system adequacy for the assortment of nuclear phenotypes based on chromatin texture evaluation. Acta Histochem. Cytochem. 27: 23-31.

Mello, M.L.S., Content, S., Vidal, B.C., Planding, W. and Schenck, U. (1995). Modulation of ras transformation affecting chromatin supraorganization as assessed by image analysis. Exp. Cell Res. 220: 374-382.

Oberholzer, M., Oestreicher, M., Christen, H. and Bruehlmann, M. (1996). Methods in quantitative image analysis. Histochem. Cell Biol. 105: 333-355.

Smith, P.S., Parkinson, I.H. and Leong, A.S.-Y. (1996). Principles of ploidy analysis by static cytometry. J. Clin. Pathol.: Mol. Pathol. 49: 104-111.

Vidal, B.C. (1984). Polyploidy and nuclear phenotypes in salivary glands of the rat. Biol. Cell 50: 137-146.

Vidal, B.C. (1992). Human renal carcinoma: Feulgen-DNA area and chromatin condensation determined by scanning cytometry. Rev. Bras. Genét. 15: 945-961.

Vidal, B.C., Schlueter, G. and Moore, G.W. (1973). Cell nucleus pattern recognition: influence of staining. Acta Cytol. 17: 510-521.

Vidal, B.C., Silva, W.J. and Strikis, P.C. (1984). Nuclear phenotypes and DNA content of root cells of Zea mays mays, Zea diploperennis and of a mazoid hybrid. Cell. Mol. Biol. 30: 11-22.

Vidal, B.C., Planding, W., Mello, M.L.S. and Schenck, U. (1994). Quantitative evaluation of AgNOR in liver cells by high-resolution image cytometry. Anal. Cell. Pathol. 7: 27-41.

 

 

* Conference presented at the 42nd National Congress of Genetics. September 4-7,1996, Caxambu, MG, Brasil.