Full text in pdf format
Changes in N2 fixation in Stylosanthes scabra derived from tissue culture
Maria José ValariniI; Ivan! Pozar OtsukI; Maria Lucia Carneiro VieiraII
IInstituto de Zootecnia de Nova Odessa, Caixa Postal 60, 13460-000 Nova Odessa, SP.
IIDepartamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz"/USP, Caixa Postal 83, 13400-970 Piracicaba, SP, Brasil. Send correspondence to M.L.C.V.
ABSTRACT
Plants were regenerated from leaf-derived callus culture of Stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. A total of 168 regenerants were planted out in Leonard jars in a complete randomized design. Nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot N content and acetylene reduction activity. The results showed higher variation in the regenerants than in controls not submitted to tissue culture. Significant differences were found for all nitrogen fixation related-traits.
Keywords: N2 fixation; Stylosanthes; tissue culture.
REFERENCES
Bussab, W.O., Andrade, D.F. and Myazaky, E.S. (1990). Introdução à Análise de Agrupamentos. In: Simpósio Nacional de Probabilidade e Estatística, 9. IME/USP, São Paulo.
Consoli, L., Vieira, M.L.C., Souza Jr., C.L. and Garcia, A.A.F. (1996). Tissue culture effects on quantitative traits in Stylosanthes guianensis. Braz. J. Genet. 19: 469-474.
Dudley, M.E. and Long, S.R. (1989). A non-nodulating alfafa mutant displays neither root hair curling nor early cell division in response to Rhizobium meliloti. Plant Cell 1: 65-72.
Kaeppler, S.M. and Phillips, R.L. (1993). Tissue culture-induced DNA methylation variation in maize. Proc. Nat. Acad. Sci. LISA 90: 8773-8776.
Larkin, P.J. and Scowcroft, W.R. (1981). Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197-214.
Mardia, K.V., Kent, J.T. and Bibby, J.M. (1979). Multivariate Analysis. Academic Press, London.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
Norris, D.O. and Date, R.A. (1976). Legume Bacteriology. In: Tropical Pasture Research. Principles and Methods. C.A.B. (Commonw. Bur. Pastures Field Crops Hurley Berkshire. Bull.) 51: 134-174.
Phillips, R.L., Kaepler, S.M. and Peschke, V.M. (1990). Do we understand somaclonal variation? In: VIIth International Congress on Plant Tissue Cell Culture Proceedings. Kluwer, Dordrecht, pp. 131-141.
Smulders, M.J.M., Rus-Kortekaas, W. and Vosman, B. (1995). Tissue culture-induced DNA methylation polymorphisms in repetitive DNA of tomato calli and regenerated plants. Theor. Appl. Genet. 91: 1257-1264.