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Milk protein polymorphisms in Brazilian Zebu cattle*

 

 

lvana Tramontina da Silva; Marco Antonio Del Lama

Departamento de Genética e Evolução, Universidade Federal de São Carlos, Rodovia Washington Luiz, km 235, 13565-905 São Carlos, SP, Brasil. Send correspondence to M.A.D.L.

 

 

ABSTRACT

Five bovine milk protein polymorphisms were studied in Zebuine cattle raised in Brazil, through horizontal electrophoresis on starch gel containing urea and 2-mercaptoethanol, using basic and acidic buffer systems. Allelic frequencies for a-La, b-Lg, as1-Cn, b-Cn and k-Cn loci were estimated in six Gyr herds (N = 283), six Guzerat herds (N = 205), one Nelore herd (N = 17) and one Sindi herd (N = 22), all from Sãο Paulo or Minas Gerais State, Brazil. Genotypic frequencies observed for each locus and breed studied are in accordance with the assumption of genetic equilibrium, demonstrating absence of high inbreeding levels for the breeds tested. The Fsτ value found indicated significant genetic differentiation among breeds; however, the Gyr and Guzerat herds showed significantly different gene frequencies. Genetic distance estimates among zebuine breeds studied and the Holstein breed, taken as a reference for a taurine breed, showed strong differences between these two racial groups.

Keywords: Milk protein; Zebu cattle.

 

 

REFERENCES

Aleandri, R., Butazzoni, L.G., Schneider, J.C., Caroli, A. and Davali, R. (1990). The effects of milk protein polymorphisms on milk components and cheese-producing ability. J. Dairy Sci. 73: 241-255.

Aschaffenburg, R. (1963). Milk protein polymorphisms. In: Man and Cattle (Mourant, A.E. and Zeuner, F.E., eds.). Royal Anthropological Institute of Great Britain and Ireland, Occasional Paper, Number 18, pp. 50-54.

Aschaffenburg, R. (1968). Reviews of the progress of dairy science. J. Dairy Sci. 35: 447-460.

Aschaffenburg, R. and Drewry, J. (1957). Genetics of beta-lactoglobulins of cow's milk. Nature 180: 376-378.

Aschaffenburg, R. and Michalak, W. (1968). Simultaneous phenotyping procedure for milk proteins - Improved resolution of the b-lactoglobulins. J. Dairy Sci. 51: 1849.

Aschaffenburg, R., Sen, A. and Thompson, M.P. (1968). Genetic variants of casein in Indian and African zebu cattle. Comp. Biochem. Physiol. 25: 117-184.

Baker, C.M.A. and Manwell, C. (1980). Chemical classification of cattle. I. Breed groups. Anim. Blood Groups Biochem. Genet. 11: 127-150.

Bhattacharya, S.D., Roychoudhury, A.K., Sinha, N.K. and Sen, A. (1963). Inherited a-lactalbumin and b-lactoglobulin polymorphism in Indian zebu cattle. Comparison

of zebu and buffalo a-lactalbumins. Nature 197: 797-799. Cavalli-Sforza, L.L. and Edwards, A.W.F. (1967). Phylogenetic analyses: models and estimation procedures.Evolution 21: 550-570.

Chianese, L., Di Luccia, A., Mauriello, R., Ferrara, L., Zehender, G. and Addeo, F. (1988). Polimorfismo biochimico delle protein del latte in bovine di razza Podolica. Zoot. Nutr. Anim. 14: 189-197.

Del Lama, S.N. (1992). Caracterização genética das raças zebuínas criadas no Brasil através de polimorfismos proteicos e grupos sanguíneos. Doctoral thesis, Faculdade de Medicina de Ribeirãο Preto. Universidade de São Paulo. SP, Brazil.

Del Lama, S.N. and Zago, M.A. (1996). Identification of the K-casein and b-lactoglobulin genotypes in Brazilian Bos indicus and Bubalus bubalis populations. Braz. J. Genet. 19: 73-78.

Del Lama, S.N., Del Lama, M.A., Mestriner, M.A. and Mortari, N. (1992). Peptidase B polymorphism in cattle erythrocytes. Biochem. Genet. 30: 247-255.

Grosclaude, F. (1988). Le polymorphisme génétique des principales lactoprotéines bovines. INRA Prod. Anim. 1: 5-17.

Grosclaude, F., Mahé, M.-F. and Mercier, J.C. (1974). Comparaison du polymorphisme génétique des lactoprotéines du zébu et des bovins. Ann. Génet. Sél. Anim. 6: 305-329.

Grosclaude, F., Mahé, M.-F., Brignon, G., Di Stasio L. and Jeunet, R. (1987). A Mendelian polimorphism underlying quantitative variations of goat as2-casein. Genét. Sél. Evol. 19: 399-412.

Hines, H.C., Kiddy, C.A., Brum, E.W. and Arave, C.W. (1969). Linkage among cattle blood and milk polymorphisms. Genetics 62: 401-409.

Jakob, Ε. (1994). Genetischer polymorphismus der milchproteine. I. Die genetischen varianten. Schweiz. Milchwirstsch. Forsch. 23: 3-11.

Lopes, M.A.B. and Resende, E.M. (1984). ABCZ: 50 Anos de História e Εstόrias. Editora Associação Brasileira dos Criadores de Zebu, Uberaba, MG, Brasil, pp. 239.

Marziali, A.S. and Ng-Kwai-Hang, K.F. (1986). Effects of milk composition and genetic polymorphism on cheese composition. J. Dairy Sci. 69: 2533-2542.

Mortari, N. (1989). Estudo de tipos sanguíneos em bovinos selecionados para corte da raça Gir (Bos indicus) criada no Brasil. Doctoral thesis, UNICAMP, Campinas, SP.

Nei, Μ. (1977). F-statistics and analysis of gene diversity in subdivided populations. Ann. Hum. Genet. 41: 225-233.

Nei, Μ. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.

Peterson, R.F. and Kopfler, F.C. (1966). Detection of new types of b-casein by polyacrylamide gel electrophoresis at acid pH: a proposed nomenclature. Biochem. Biophys. Res. Commun. 22: 388-392.

Rando, A., Di Gregorio, P. and Masina, P. (1988). Identification of bovine K-casein genotypes at DNA level. Anim. Genet. 19: 51-54.

Rogne, S., Lien, S., Vegarud, G., Steine, T., Langsrud, T. and Alestrom, P. (1989). A method for K-casein genotyping of bulls. Anim. Genet. 20: 317-321.

Sabour, M.P., Lin, C.Y., Keough, A., Mechanda, S.M. and Lee, A.J. (1993). Effects of selection practiced on the frequencies of K-casein and b-lactoglobulin genotypes in Canadian artificial insemination bulls. J. Dairy Sci, 76: 274-280.

Santiago, A.A. (1985). 0 Zebu na India, no Brasil e no Mundo. Instituto Campineiro de Ensino Agricola, Campinas, SP, Brasil, pp. 744.

Singh, Η. and Bhat, P.N. (1980). b-lactoglobulin polymorphism in indigenous cattle. Indian J. Anim. Sci. 50: 932-937.

Singh, Η. and Bhat, P.N. (1981a). b-casein polymorphism in indigenous cattle. Indian J. Anim. Sci. 51: 11-16.

Singh, H. and Bhat, P.N. (1981b). Gene differentiation in Indian cattle. Indian J. Anim. Sci. 51: 267-270.

Singh, H.P. and Khanna, N.D. (1972). Milk protein polymorphism in Hariana cattle. A note. Indian J. Anim. Sci. 42: 468-469.

Swofford, D.L. and Selander, R.B. (1981). BIOSYS-1: a FORTRAN program for the comprehensive analyses of electrophoretic data in population genetics and systematics. J. Hered. 72: 281-283.

Vienne, D. and Demerval, C. (1985). Mesures de la divergence génétique. In: Le Distances Genetics: Estimation et Applications. Le Font Blanne & Vienne eds., Paris, pp. 181.

Villares, J.B. (1979). Novas perspectivas para produção de leite no Brasil. Rev. Criadores 49: 21-32.

Weir, B.S. (1990). Genetic Data Analysis: Methods for Discrete Population Genetic Data. Sinauer, Sunderland, pp. 377.

Weir, B.S. and Cockerham, C.C. (1984). Estimating F-statistics for the analysis of population structure. Evolution 38: 1358-1370.

Wilkins, R.J. and Kuys, Y.M. (1992). Rapid b-lactoglobulin genotyping of cattle using the polymerise chain reaction. Anim. Genet. 23: 175-176.

Workman, P.L. and Niswander, J.D. (1970). Population studies on southwestern indian tribes. II. Local genetic differentiation in the Papago. Am. J. Hum. Genet. 22:24-29.

 

 

* Part of a thesis presented by I.T.S. to the Center of Biological Sciences and Health, Universidade Federal de São Carlos, in partial fulfillment of the requirements for a Master's degree.