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Transient expression of a reporter gene introduced by bioballistic bombardment into Racosperma mangium (Leguminosae family) tissues
M. QuoirinI; F. AragãoII; E. RechII; D.E. De OliveiraIII
IDepartamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná (UFPR), Caixa Postal 19031, 81531-970 Curitiba, PR, Brasil. Phone/Fax: 55 41 2662042. E-mail: quoirin@bio.ufpr.br. Send correspondence to M.Q.
IICentro Nacional de Recursos Genéticos (CENARGEN), EMBRAPA, Brasília, DF, Brasil.
IIIDepartamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
ABSTRACT
We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues.
Keywords: reporter gene; bioballistic; Leguminosae.
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