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Two point deterministic model for acquisition of in vitro pollen grain androgenetic capacity based on wheat studies*
Magali Ferrari GrandoI; Maria Irene B. de Moraes-Fernandes II
IUniversidade de Passo Fundo, Caixa Postal 566, 99001-970 Passo Fundo, RS, Brasil.
ICentro Nacional de Pesquisa em Trigo, EMBRAPA, Caixa Postal 569, 99001-970 Passo Fundo, RS, Brasil. Send correspondence to M.I.B.M: F.
ABSTRACT
This article discusses, from the standpoint of cellular biology, the deterministic and indeterministic androgenesis theories. The role of the vacuole and of various types of stresses on deviation of the microspore from normal development and the point where androgenetic competence is acquired are examined. Based on extensive literature review and data on wheat studies from our laboratory, a model for androgenetic capacity of pollen grain is proposed. A two point deterministic model for in vitro androgenesis is our proposal for acquisition of androgenetic potential of the pollen grain: the first switch point would be early meiosis and the second switch point the uninucleate pollen stage, because the elimination of cytoplasmatic sporophytic determinants takes place at those two strategic moments. Any abnormality in this process allowing the maintenance of sporophytic informational molecules results in the absence of establishment of a gametophytic program, allowing the reactivation of the embryogenic process.
Keywords: deterministic model; pollen grain; androgenetic capacity.
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* Part of a thesis presented by M.F.G. to the Graduate Course of Genetics of the Universidade Federal do Rio Grande do Sul (UFRGS) in partial fulfillment for the requirements of the Master's degree.