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Isozyme markers and genetic variability in three species of Centrosema (Leguminosae)
Maria Isabel de O. PenteadoI; Pedro GarciaII; Marcelino Pérez de la VegaII
ICNPGC/EMBRAPA, Rod. BR 262, km 4, Caixa Postal 154, 79002-970
Campo Grande, MS, Brasil.
IIArea de Genética, Facultad de Biologia, Universidad de León, 24071 Left, Spain. Send correspondence to M.P.V.
ABSTRACT
Isozyme patterns and their genetic control in three Centrosema species are described. Seven isozymatic systems (aspartate aminotransferase, glucose-6-phosphate isomerase, phosphoglucomutase, anodal peroxidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and isocitrate dehydrogenase) were studied in 18 populations and several breeding lines of C. acutifolium, C. brasilianum and C. pubescens, using starch gel electrophoresis techniques. All systems, except glucose-6-phosphate isomerase, are described for the first time in these species. A total of 17 isozyme loci were scored; this represents the largest set of Mendelian loci known up to now in Centrosema species. Isozyme polymorphism and variability within and between populations and species were relatively high and allowed discrimination among species.
Keywords: Isozyme; genetic variability; Leguminosae.
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