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Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
Ana Maria Waldschmidt; Tânia Maria Fernandes Salomão; Everaldo Gonçalves de Barros; Lúcio de Antônio Oliveira Campos
Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG, Brasil. Send correspondence to A.M.W.
ABSTRACT
The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvae.
Keywords: genomic DNA; Melipona quadrifasciata; Hymenoptera; Meliponinae.
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