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Benzidine- and diaminobenzidine-induced micronuclei in mice after intraperitoneal and oral single or multiple treatment
Maria Clara Gimmler-Luz; Heloisa Helena Rodrigues de Andrade; Andréa Tozzo Marafon-Bayer
Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-970 Porto Alegre, RS, Brasil. Fax: 5551-336-2011. Send correspondence to M.C.G.-L.
ABSTRACT
The capacity of benzidine and its derivative diaminobenzidine to induce micronuclei in bone marrow polychromatic erythrocytes was tested in two mouse strains, C57BL/6 and BALB/c, after intraperitoneal injection or gavage on one, two or three successive days. Results observed after multiple intraperitoneal dosages were inconclusive for both compounds, when tested in C57BL/6 mice. On the other hand, benzidine induced a marked increase in the micronucleated polychromatic erythrocyte frequency after a single intraperitoneal treatment in BALB/c mice. Oral treatment with both chemicals had a genotoxic effect in both mouse strains, although in BALB/c mice a significant increment in micronucleated cell frequency was observed only after two or three consecutive doses. We conclude that diaminobenzidine has a genotoxic activity comparable to benzidine, presenting specific strain and administration route effects.
Keywords: micronuclei; mice; benzidine; diaminobenzidine.
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