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Amylase and protease secretionin recombinant strains of Metarhizium anisopliae var. anisopliaefollowing parasexual crosses
M.C. Valadares-InglisI; J. L. AzevedoII
ICENARGEN/EMBRAPA, Caixa Postal 02372, 70770-900 Brasília, DF, Brasil. Send correspondence to M.C.V-I.
IIEscola Superior de Agricultura "Luiz de Queiroz", Departamento de Genética, Caixa Postal 83, 13400-970 Piracicaba, SP, Brasil.
ABSTRACT
Parasexual crosses were made between auxotrophic mutants of Metarhizium anisopliae var. anisopliae. Analysis of recombinants for secretion of amylases and proteases demonstrated that stable prototrophic recombinants have similar levels of enzyme secretion. Unstable prototrophic recombinants varied in amylase production, and segregants originating from these varied in protease secretion. Auxotrophic recombinants showed intense variation in secretion of amylases and proteases although there was no correlation between auxotrophic markers and enzyme production. There were significant differences in enzyme secretion between recombinants and parentals.
Keywords: Metarhizium; Amylase; protease; parasexual crosses.
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