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Cephalosporin C production and genetic improvement of the fungus Acremonium chrysogenum based on morphological mutant isolation*
Airton VialtaI; Cleide Ferreira CataniII; Renato Bonatelli JuniorIII†; João Lúcio AzevedoIV
IInstituto de Tecnologia de Alimentos, Av. Brasil, 2880, Caixa Postal 139, 13073-001 Campinas, SP, Brasil. Send correspondence to A.V.
IIDepartamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, Caixa Postal 6109, 13083-970 Campinas, SP, Brasil.
IIIDepartamento de Genética e Evolução, Universidade Estadual de Campinas, Caixa Postal 6109, 13083-970 Campinas, SP, Brasil.
IVDepartamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Caixa Postal 83, 13418-900 Piracicaba, SP, Brasil.
†Deceased.
ABSTRACT
C-10 strain of the fungus Acremonium chrysogenum produced about 1.5 g/1 of cephalosporin C, a yield that was much lower than expected according to the literature. This discrepancy, confirmed by exhaustive tests, showed that C-10 strain lost its high cephalosporin C production ability, probably due to genetic events. Strain improvement, based on isolation of morphologically compact mutant colonies, confirmed that this is a useful procedure for isolation of strains with increased titer. About 2% of tested colonies produced significantly more cephalosporin C than the original strain.
Keywords: Acremonium; Cephalosporin C; morphological mutant.
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