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Sequence analysis of thecatalytic domain of a Metarhizium anisopliae chitinase
Maria Cléria Valadares-InglisI; Peter W. InglisI; John F. PeberdyII
ICentro National de Recursos Genéticos e Biotecnologia (CENARGEN/EMBRAPA), Caixa Postal 02372, 70849-970 Brasília, DF, Brasil. Tel: +55 61 340 3575. Fax: +55 61 340 3573. E-mail: cleria@cenargen.embrapa.br. Send correspondence to M.C.V.-I.
IIDepartment of Life Science, University of Nottingham, Nottingham NG7 2RD, UK.
ABSTRACT
A 375-bp DNA fragment from Metarhizium anisopliae strain CG32 genomic DNA was amplified by PCR using chitinase-specific concensus primers. The deduced M. anisopliae peptide sequence demonstrated high similarity to the catalytic domains of chitinase genes from Aphanocladium album and Trichoderma harzianum. However, two introns present in the aligned A. album and T. harzianum genomic sequences were not detected in the M. anisopliae sequence. A comparison was made between this Metarhizium chitinase sequence and that of other fungi and bacteria. Possible phylogenetic relationships are discussed.
Keywords: genomic sequences; Metarhizium.
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