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Interphase cytogenetics using fluorescence in situ hybridization: an overview of its application to diffuse and solid tissue
Christine HackelI; Marileila Varella-GarciaII
IDepartamento de Genética Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Caixa Postal 6111, 13083-970 Campinas, SP, Brasil. Fax: 55-19-2393114. Send correspondence to C.H.
IICytogenetics Core, University of Colorado Cancer Center, UCHSC, Denver, CO 80262, USA.
ABSTRACT
Interphase cytogenetics, utilizing fluorescence in situ hybridization (FISH) techniques, has been successfully applied to diffuse and solid tissue specimens. Most studies have been performed on isolated cells, such as blood or bone marrow cells; a few have been performed on cells from body fluids, such as amniotic fluid, urine, sperm, and sputum. Mechanically or chemically disaggregated cells from solid tissues have also been used as single cell suspensions for FISH. Additionally, intact organized tissue samples represented by touch preparations or thin tissue sections have been used, especially in cancer studies. Advantages and pitfalls of application of FISH methodology to each type of specimen and some significant biological findings achieved are illustrated in this overview.
Keywords: fluorescence; Interphase; FISH methodology.
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