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Intra and interspecific variability of in vitro culture response in Lycopersicon (tomatoes)
Guillermo Pratta; Roxana Zorzoli; Liliana Amelia Picardi
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cátedra de Genética, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, CC 14 - 2123 Zavalla, Santa Fe, Argentina. Tel/Fax: 54-41/ 970080/ 970085. Send correspondence to G.P.
ABSTRACT
Intra and interspecific variability was measured in the genus Lycopersicon for the traits: productivity rate (PR, total number of regenerated shoots/total number of cultures), regeneration percentage (%R, number of cultures regenerating shoots or primordia/total number of cultures) and callus percentage (%C, number of cultures only producing callus/total number of cultures). Leaf explants from various genotypes of L. esculentum, L. esculentum var. cerasiforme, L. pimpinellifolium and L. peruvianum were placed on Murashige and Skoog (Physiol. Plant. 15: 473-493, 1962) medium + 0.175 mg/1 IAA + 2.25 mg/1 BA. Significant differences among species and among genotypes within the same species were found, while genotypes from different species showed similar responses.
Keywords: Lycopersicon; variability; productivity.
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