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Callus induction and plant regeneration by Brazilian triticale and wheat genotypes
A.L.C. Dornelles; F.I.F. Carvalho; LC. Federizzi; C.L. Handel; F. Bered; M.E.B. Sordi; F. Schneider
Departamento de Horticultura e Silvicultura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 7712, 91501-970 Porto Alegre, RS, Brasil. Send correspondence to A.L.C.D.
ABSTRACT
In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/1, and MS medium containing 4.0 mg 2,4D/1. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.
Keywords: Callus; plant regeneration; triticale; wheat.
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