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A comparative study of eleven protein systems in tamarins, genus Saguinus(Platyrrhini, Callitrichinae)
Carla Meireles, Iracilda SampaioI; Horacio SchneiderI; Stephen F. FerrariI; Adelmar F. Coimbra-FilhoII; Alcides PissinattiII; Maria P. C. SchneiderI
IDepartamento de Genética, Centro de Ciências Biológicas, Universidade Federal do Pará, Campus Universitário do Guamá, Caixa Postal 8607, 66075-900 Belém, PA, Brasil. Tel: (091) 211-1627, Fax: (091) 211-1568. Send correspondence to M.P.C.S.
IICentro de Primatologia do Rio de Janeiro, CPRJ FEEMA, Rio de Janeiro, Brasil.
ABSTRACT
The genetic variability of six tamarin taxa, genus Saguinus, was analyzed comparatively using protein data from eleven systems coded by 15 loci. S. fuscicollis weddelli and S. midas midas were the most polymorphic taxa, and S. bicolor the least. The results of the phylogenetic analyses (UPGMA and neighbor-joining) and the genetic distances between taxa were generally consistent with their geographic and probable phylogenetic relationships. Analyses of the S. bicolor and S. midas populations suggested that they represent no more than three subspecies of a single species, S. midas, with the bicolor forms belonging to a single subspecies, S. midas bicolor. If supported by additional studies, this would have important implications for the conservation of the bicolor form, which is endangered with extinction. The genetic similarity of S. fuscicollis and S. mystax was also consistent with their geographical and morphological proximity, although more data from a larger number of taxa will be required before the taxonomic relationships within the genus can be defined.
Keywords: Platyrrhini; protein systems; phylogeny; genetic variability.
REFERENCES
Coimbra-Filho, A.F. (1990). Sistemática, distribuição geográfica e situação atual dos símios brasileiros (Platyrrhini, Primates). Rev. Bras. Biol. 50: 1063-1079.
Dantas, S.M.M. (1994). Estudos citogenéticos em oito subespécies do gênero Saguinus (Primates). Master's thesis, Universidade Federal do Pará, Belém, Brasil.
de Vivo, M. (1991). Taxonomia de Callithrix Erxleben, 1777 (Callitrichidae, Primates). Fundação Biodiversitas, Belo Horizonte, Minas Gerais, Brasil, pp. 105.
Egler, S.G. (1993). First field study of the pied tamarin; Saguinus bicolor bicolor. Neotropical Primates 1: 13-14.
Ferrari, S.F. (1993). The adaptive radiation of Amazonian callitrichids (Primates, Platyrrhini). Evol. Biol. 7: 81-103.
Forman, L, Kleiman, D.G., Bush, R.M., Dietz, J.M., Ballou, J.D., Phillips, L.G., Coimbra-Filho, A.F. and O'Brien, S.J. (1986). Genetic variation within and among lion tamarins. Am. J. Phys. Anthropol. 71: 1-11.
Franco, M.H.L.P. and Salzano, F.M. (1985). A comparative study of albumin variants found in Brazil. Human Hered. 35: 34-38.
Hanihara, T. and Natori, M. (1987). Preliminary analysis for numerical taxonomy of the genus Saguinus based on dental measurements. Primates 28: 517-523.
Harris, H. and Hopkinson, D.A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics. North-Holland Publishing Company, Amsterdam.
Hershkovitz, P. (1977). Living New World Monkeys (Platyrrhini) with an Introduction to Primates. Vol. I. University of Chicago Press, Chicago.
Hershkovitz, P. (1979). Races of the emperor tamarin, Saguinus imperator Goeldi (Callitrichidae, Primates). Primates 20: 277-287.
Hershkovitz, P. (1982). Subspecies and geographic-distribution of black-mantle tamarins Saguinus nigricollis Spix (Primates: Callitrichidae). Proc. Biol. Soc. Wash. 95: 647-656.
Hershkovitz, P. (1990). Titis, New World monkeys of the genus Callicebus (Cebidae, Platyrrhini): a preliminary taxonomic review. Fieldiana Zool. 55: 1-109.
Kumar, S., Tamura, K. and Nei, M. (1993). MEGA - Molecular Evolutionary Analysis. Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park.
Melo, A.C.A., Sampaio, M.I.C., Schneider, M.P.C. and Schneider, H. (1992). Biochemical diversity and genetic distance in two species of the genus Saguinus. Primates 33: 217-225.
Mittermeier, R.A., Rylands, A.B. and Coimbra-Filho, A.F. (1988). Systematics: species and subspecies - an update. In: Ecology and Behavior of Neotropical Primates (Mittermeier, R.A., Rylands, A.B., Coimbra-Filho, A.F. and Fonseca, G.A.B., eds.). Vol. 2. World Wildlife Fund., Washington, D.C., pp. 13-75.
Mittermeier, R.A., Schwarz, M. and Ayres, J.M. (1992). A new species of marmoset, genus Callithrix Erxleben, 1777 (Callitrichidae, Primates) from the Rio Maués region, State of Amazonas, central Brazilian Amazonia. Goeldiana Zoologia 14: 1-17.
Nagai, A., Shimaoka, T., Lee, S.C. and Ikemoto, S. (1986). Genetic markers in the blood of three species of tamarins (Saguinus mystax, S. labiatus and S. oedipus). Exp. Anim. 35: 375-380.
Nagamachi, C.Y. (1995). Relações cromossômicas e análises filogenética e de agrupamento na família Callitrichidae (Platyrrhini, Primates). Doctoral thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Natori, M. and Hanihara, T. (1992). Variations in dental measurements between Saguinus species and their systematic relationships. Folia Primatol. 58: 84-92.
Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
Nei, M. (1987). Molecular Evolutionary Genetics. Columbia University Press, New York.
Reed, T.E. and Schull, W.J. (1968). A general maximum likelihood estimation program. Am. J. Hum. Genet. 20: 579-580
Rylands, A.B., Coimbra-Filho, A.F. and Mittermeier, R.A. (1993). Systematics, geographic distribution, and some notes on the conservation status of the Callitrichidae. In: Marmosets and Tamarins: Systematics, Ecology, and Behaviour (Rylands, A.B., ed.). Oxford University Press, Oxford, pp. 11-77.
Saitou, N. and Nei, M. (1987). The neighbor-joining method; a new method for reconstructing phylogeneic trees. Mol. Biol. Evol. 4: 406-425.
Sampaio, M.I.C. and Schneider, M.P.C. (1986). Electrophoretic studies of a natural population of Chiropotes satanas (Cebidae, Platyrrhini) from the Amazonian region. Rev. Bras. Genet. 9: 67-74.
Sampaio, M.I.C., Schneider, M.P.C., Salzano, F.M. and ' Barroso, C.M.L. (1986). Esterase D and carbonic anhydrase 2 in a natural population of Cebus apella from Brazil. Primates 27: 363-367.
Sampaio, M.I.C., Barroso, C.M.L., Silva, B.T.F., Seuanez, H., Matayoshi, T., Howlin, E., Nazassi, N., Nagle, C. and Schneider, H. (1991). Genetic variability in Cebus apella paraguayanus: biochemical analysis of seven loci and variation in glyoxalase I (E.C. 4.4.1.5). Primates 32: 105-109.
Schneider, H., Sampaio, M.I.C., Schneider, M.P.C., Ayres, J.M., Barroso, C.M.L., Hamel, A., Silva, B.T.F. and Salzano, F.M. (1991). Coat color and biochemical variation in Amazonian wild population of Alouatta belzebul. Am. J. Phys. Anthropol. 85: 85-93.
Schneider, H., Schneider, M.P.C., Sampaio, I., Hark! M.L., Stanhope, M., Czelusniak, J. and Goodman, M. (1993). Molecular phylogeny of the New World monkeys (Platyrrhini, Primates). Mol. Phylog. Evol. 2: 225-242.
Schneider, M.P.C. (1988). Variação protéica em primatas da Amazônia e seu significado evolutivo. Doctoral thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Shows, T.B., Alper, C.A., Bootsma, D., Dorf, M., Douglas, T., Huisman, T., Kit, S., Klinger, H.P., Kozak, C., Lalley, P.A., Lindsley, D., Mcalpine, P.J., Mcdougall, J.K., Meera Khan, P., Meisler, M., Morton, N.E., Opitz, J.M.,
Partridge, C.W., Payne, R., Roderick, T.H., Rubinstein, P., Ruddle, F.H., Shaw, M., Spranger, J.W. and Weiss, K. (1979). International system for human gene nomenclature (ISGN). Cytogenet. Cell Genet. 25: 96-116.
Thorington Jr., R.W. (1988). Taxonomic status of Saguinus tripartitus (Milne-Edwards, 1878). Am. J. Primatol. 15: 367-371.
Thorpe, J.P. (1982). The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Ann. Rev. Ecol. Syst. 13: 139-168.