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A new allele of peptidase-B in cattle

Maria Aparecida Cassiano Lara^I; Eucleia Primo B. Contel^II

^ISeção de Reprodução e Inseminação Artificial, Instituto de Zootecnia, Caixa Postal 60,13141-000 Nova Odessa, SP, Brasil.
^IIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, USP, 14049-900 Ribeirão Preto, SP, Brasil. Send correspondence to E.P.B.C.

ABSTRACT

Electrophoretic analyses of peptidase-B were carried out on red cell hemolysates from Holstein, Mantiqueira and Gyr cattle, using cornstarch, known in Brazil as Penetrose-30. We describe a new peptidase-B allele, denoted Pep-B³, in Mantiqueira cattle, belonging to the Bos taunts group, which are the result of a cross of native cattle of Portuguese origin introduced in Brazil during colonial times (16th century) with Holstein and Caracu cattle. The genetic control of peptidase-B was determined by typing parents and progeny segregating for all three alleles, confirming that peptidase B is controlled by a single autosomal locus with three codominant alleles, denoted Pep-B¹, Pep-B² and Pep-B³. The use of the citrate-phosphate buffer system, at pH 5.9, on 14% gel, under the electrophoretic conditions standardized in this study permitted good visualization of all peptidase-B variants.

Keywords: peptidase B; Electrophoretic analyses; Mantiqueira cattle.

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