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Isoenzymatic characterization of hybrids between Leucaena leucocephala and Leucaena diversifolia ssp. diversifolia grown in Rio Grande do Sul (Southern Brazil)

Maria Teresa Schifino-Wittmann; Liane Helena Cardoso de Freitas; Carine Simioni

Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Caixa Postal 776, 91501-970 Porto Alegre, RS, Brasil. Send correspondence to M.T.S.-W.

ABSTRACT

Esterase (EST), superoxidedismutase (SOD) and malic enzyme (ME) isozyme patterns were analyzed in leucaena leucocephala and tetraploid L. diversifolia ssp. diversifolia and in F2. F3. and F4 hybrid plants. No variability was detected in ME, all individuals presenting only one and the same band (rm = 0.17). For SOD clearly different patterns in L. leucocephala (rm = 0.32, 0.40, 0.70) vs. L. diversifolia (rm = 0.12, 0.35, 0.39, 0.72) were found. Hybrid plants presented combined patterns and some of them had a new band (rm = 0.50), absent in the parental species. In EST a great variation within species and hybrids was verified. Up to nine a(rm = 0.30, 0.35, 0.40, 0.45, 0.50, 0.55, 0.62, 0.75 and 0.90) and four b(rm = 0.10, 0.20, 0.25, 0.28) bands were detected; some hybrids had bands absent in the parental species. However, no distinctive EST patterns for the species could be established.

Keywords: isoenzymatic characterization; hybrids; Leucaena leucocephala; Leucaena diversifolia.

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