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Tissue culture effects on quantitative traits in Stylosanthes guianensis (Leguminosae)

Luciano Consoli; Maria Lúcia Carneiro Vieira; Cláudio Lopes de Souza Jr.; Antônio Augusto Franco Garcia

Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Caixa Postal 83, 13418-900 Piracicaba, SP, Brasil. Send correspondence to M.L.C.V.

ABSTRACT

Sixty-three tissue culture-derived progenies (R₁) and 53 progenies from the original population (O₁) of Stylosanthes guianensis were assessed for six traits of interest for pasture. An increase of 50.7%, 102.2% and 61.9% in the genetic variability was observed, respectively, for the traits basal area (BA), basal diameter (BD) and plant vigor (PV) in the regenerated population. A decrease in the genetic variability of 45.5% for fresh matter yield (FMY) and 32.9% for dry matter yield (DMY) was noted in the regenerated populations, while no difference was found for main stem height (MSH). Except for MSH, the means of all traits in the regenerated population were lower than those in the original population. Thus, the expected means of R(m_sr) population were lower than those of control (m_so) for the traits FMY (m_so = 306.37 and m_sr = 248.76 g/plant) and DMY (m_so = 72.12 and m_sr = 61.61 g/plant).

Keywords: tissue culture; Stylosanthes guianensis.

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