Full text in pdf format

Genetic diversity of the common bean Phaseolus vulgaris L. determined by DNA-based molecular markers

Maria J.V. Vasconcelos^I; Everaldo G. de Barros^II; Maurilio A. Moreira^II; Clibas Vieira^III

^ICentro Nacional de Pesquisa de Milho e Sorgo (CNPMS/ EMBRAPA), 35701-970 Sete Lagoas, MG, Brasil
^IINúcleo de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG, Brasil. Send correspondence to E.G.B.
^IIIDepartamento de Fitotecnia, Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG, Brasil

ABSTRACT

We selected 28 potential common bean (Phaseolus vulgaris L.) progenitors to determine their genetic diversity using RAPD technique. They were divided into two groups based on their type of storage protein (phaseolin); 18 of them had type "S" phaseolin and the other 10 had type "T" phaseolin. DNA samples of all the cultivars were amplified in vitro with oligonucleotide primers of random sequences. A total of 276 amplification products, 144 of them being polymorphic, were obtained with 45 different primers. Multivariate analysis of these data divided the cultivars into two groups and, in addition, allowed a more precise intra and intergroup distinction.

Keywords: genetic diversity; bean; Phaseolus vulgaris L.; molecular markers.

REFERENCES

Araújo, G.A.A., Vieira, C. and Souza Filho, B.F. (1991). 'Ouro Negro' Nova variedade de feijão-preto para os estados de Minas Gerais e Rio de Janeiro. EPAMIG, Comunicado Técnico No. 1, pp. 1-2.

Cruz, C.D. and Viana, J.M.S. (1994). A methodology of genetic divergence analysis based on sample unit projection on two-dimensional space. Rev. Bras. Genet. 17: 69-73.

FAO - Food and Agriculture Organization of the United Nations (1991). Production yearbook. Vol. 45. Rome, pp. 100-101.

Gepts, P., Osborn, T.C., Rashka, K. and Bliss, F.A. (1986). Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): Evidence for multiple centers of domestication. Econ. Bot. 40: 451-468.

Haley, S.D., Miklas, P.N., Afanador, L. and Kelly, J.D. (1994). Random amplified polymorphic DNA (RAPD) marker variability between and within gene pools of common bean. J. Amer. Soc. Hort. Sci. 119: 122-125.

Hu, J. and Quiros, C.F. (1991). Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10: 505-511.

IBGE - Instituto Brasileiro de Geografia e Estatística (1991). Anuário Estatístico do Brasil. Vol. 51. Rio de Janeiro, pp. 1024.

Keim, P., Olson, T.C. and Shoemaker, R.C. (1988). A rapid protocol for isolating soybean DNA. Soybean Genet. Newslett. 15: 150-152.

Koinange, E.M.K. and Gepts, P. (1992). Hybrid weakness in wild Phaseolus vulgaris L. J. Hered. 83: 135-139.

Laemmli, U.K. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685.

Nei, M. and Li, W.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273.

Pereira, P.A.A. and Souza, C.R.B. (1992). Tipos de faseolina em raças "crioulas" de feijão no Brasil. Pesq. Agrop. Bras. 27: 1219-1221.

Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Vol. 3. 2nd edn. Cold Spring Harbor Laboratory Press, New York, pp. E5-E7.

Singh, S.P., Gepts, P. and Debouck, D.G. (1991). Races of common bean (Phaseolus vulgaris, Fabaceae). Econ. Bot. 45: 379-396.

Vilarinhos, A.D., Barros, E.G., Paiva, E., Sediyama, C.S. and Moreira, M.A. (1994). Use of the random amplified polymorphic DNA technique to characterize soybean (Glycine max (L.) Merrill) genotypes. Rev. Bras. Genet. 17: 287-290.

Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218.

Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.