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Aluminum toxicity in barley (Hordeum vulgare L.) root tips^*

M.I. Eckert^I; J. Viégas^III; E.A. Osorio^I; J.B. da Silva^III; J.L. Brauner^I

SUP>IFaculdade de Agronomia Eliseu Maciel, UFPEL, Pelotas, RS, Brasil
^IIInstituto de Biologia, UFPEL, Caixa Postal 354, 96010-900 Pelotas, RS, Brasil. Send correspondence to J.V.
^IIIInstituto de Física e Matemática, UFPEL, Pelotas, RS, Brasil

ABSTRACT

The effect of aluminum (A1⁺³) on cell division in barley (Hordeum vulgare L.) root tips was studied through hydroponic cultivation in a greenhouse. Cultivars FM 404 (tolerant) and PFC 8026 (susceptible) were submitted to 0, 2, 4, and 6 ppm of Al⁺³ for two, four, six, eight and 10 days. Statistical analysis showed that the inhibition of cell division caused by aluminum was significantly lower in the tolerant cultivar kept in 2 ppm for six and eight days. The regression analyses showed, for both cultivars, a highly significant variance of percentage of cell division (PCD) with relation to aluminum concentration, and a significant variance of PCD with relation to length of exposure. Regrowth of the material treated for eight days and subsequently transplanted to an aluminum-free solution for another two days revealed a higher PCD in the tolerant cultivar at the 4 and 6 ppm concentrations. The tolerant cultivar had better PCD than the susceptible cultivar with relation to aluminum toxicity.

Keywords: aluminum toxicity; barley; root tips.

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* Part of a thesis presented by M.I.E. to Universidade Federal de Pelotas (UFPEL) in partial fulfillment of the requirements for the Master's degree.