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Mutagenicity of sodium azide in Phaseolus vulgaris L.^*

Elson Gonzaga Silva^I; Hélio Morais Barbosa^II

^IR. D. Pedro II, 57, 36404-000 Congonhas, MG, Brasil
^IIDepartamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil. Send correspondence to H.M.B.

ABSTRACT

Dormant seeds of Phaseolus vulgaris L., cv. Milionário 1732, were treated with sodium azide (SA) solutions at concentrations 0 (control), 0.04 mM, 0.12 mM, 0.36 mM, and 1.08 mM, prepared in 0.1 M phosphate buffer, pH 3. Under these conditions, SA was only weakly mutagenic, in contrast to what has been previously reported in barley. Profound metabolic disturbances were caused by the high acidity of the buffer solution, resulting in heritable phenotypic changes as found in the control treatment. The pH of the buffer solution and pregermination of seeds should be the major variables used in future experiments aiming at optimizing SA treatments of bean seeds.

Keywords: mutagenicity; sodium azide; Phaseolus vulgaris L.

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* Part of a thesis presented by E.G.S. to the Universidade Federal de Viçosa, in partial fulfillment of the requirements for the Master's degree.