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Isoenzymatic characterization of some Lathyrus L. and Vicia. L. species (Leguminosae) occurring in Rio Grande do Sul (Southern Brazil)^*

Ana Celina Gonzalez; Maria Teresa Schifino-Wittmann

Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Caixa Postal 776, 91501-970 Porto Alegre, RS, Brasil. Send correspondence to M.T.S.-W.

ABSTRACT

MDH, ME and SOD electrophoretic patterns were studied in 18 accessions of five Lathyrus and 18 accessions of nine Vicia species, using polyacrylamide gels, Scandalios (Biochem. Genet. 3: 37-79, 1969) buffer systems and specific staining solutions for each enzyme. For both genera, more than one pattern for each system for each species was found, with few exceptions. Phenograms constructed using Jaccard's similarity coefficients and the simple linkage grouping method showed that, in general, species relationships determined by isoenzymes are in accordance with taxonomic classification.

Keywords: isoenzymatic; Lathyrus L.; Vicia. L.

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* Part of a thesis presented by A.C.G. to the Universidade Federal do Rio Grande do Sul (UFRGS) in partial fulfillment of the requirements for the Master's degree.