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The metaphase and polytene chromosomes of Drosophila seriema (repleta group; mulleri subgroup)

Gustavo C.S. Kuhn^I; Alfredo Ruiz^II; Maurilio A.R. Alves^I; Fábio M. Sene^III

^IDepartamento de Biologia da Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil
^IIDepartamento de Genética y de Microbiologia, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193 Bellatera (Barcelona), España
^IIIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brasil. Send correspondence to F.M.S.

ABSTRACT

The metaphase and salivary gland chromosomes of Drosophila seriema were investigated in samples from seven different localities, which cover the known geographical range of the species. Very little chromosomal variability was found. A new karyotype was observed in two different localities and, in addition, the fifth chromosome was found to be polymorphic for a small paracentric inversion in one of the stocks. Thus this species appears to be essentially monomorphic, with low interpopulational differentiation, at least at the chromosomal level, in comparison with other species of the buzzatii complex. The phylogenetic relationships of D. seriema with its nearest relatives are discussed. Here we expand the forementioned studies of the metaphase and polytene chromosomes. In addition, we analyzed the polytene chromosomes of the hybrid larvae from the cross between D. seriema and D. borborema. This allowed a direct comparison of the chromosomal arrangements between these two species.

Keywords: metaphase; polytene chromosomes; Drosophila seriema.

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