Full text in pdf format

Cytogenetic analysis of breast carcinoma

Rommel Rodriguez Burbano^I; José Barbieri Neto^II; Paulo Meyer de Paula Philbert^III; Cacilda Casartelli^I

^IDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), 14049-900 Ribeirão Preto, SP, Brasil. Send correspondence to C.C.
^IIDepartamento de Patologia, FMRP, USP, Ribeirão Preto, SP, Brasil
^IIIDepartamento de Ginecologia e Obstetricia, FMRP, USP, Ribeirão Preto, SP, Brasil

ABSTRACT

Cytogenetic analysis was performed on six breast carcinomas. All cases had 46 as a modal chromosome number and all but one had clonal numerical alterations. The most frequent numerical alterations were the clonal monosomy of chromosomes 18 and 16, present in four and three of the six cases, respectively. The only clonal structural alteration found in one case was del(X)(q24). In one of the cases it was possible to propose the sequence of clonal evolution.

Keywords: cytogenetic analysis; breast carcinoma.

REFERENCES

Bullerdiek, J., Leuschner, E., Taquia, E., Bonk, U. and Bartnitzke, S. (1993). Trisomy 8 as a recurrent clonal abnormality in breast cancer? Cancer Genet. Cytogenet. 65: 64-67.

Callahan, R. (1987). Retrovirus and proto-oncogene involvement in the etiology of breast neoplasia. In: The Mammary Gland: Development, Regulation and Function (Neville, M.C. and Daniel, C.W., eds.). Plenum, New York, pp. 920.

Chen, L., Kurisu, W., Ljung, B., Erics-Moore II, G.D. and Smith, H.S. (1992). Heterogeneity of allelic loss in human breast cancer. J. Natl. Cancer Inst. 84: 506-510.

Cleton-Jansen, A.M., Moerland, E.W., Kuipers-Dijkshoorn, N.J., Callen, D.F., Sutherland, G.R., Hansen, B., Devilee, P. and Cornelisse, C.J. (1994). At least two different regions are involved in allelic imbalance on chromosome arm 16q in breast cancer. Genes Chromosom. Cancer 9: 101-107.

Devilee, P. and Cornelisse, C.J. (1994). Somatic genetic changes in human breast cancer. Biochim. et Biophys. Acta 1198: 113-130.

Fearon, E.R., Cho, K.R., Nigro, J.M., Kern, S.E., Simons, J.W., Ruppert, J.M., Hamilton, S.R., Preisinger, A.C., Thomas, G., Kinzler, K.W. and Vogelstein, B. (1990). A genetic model for colorectal tumorigenesis. Identification of a chromosome 18q that is altered in colorectal cancers. Science 247: 49-56.

Frei III, E. (1993). Gene deletion: a new target for cancer chemotherapy. Lancet 342: 662-664.

Goddard, A.D. and Solomon, E. (1993). Genetic aspects of cancer. Adv. Hum. Genet. 21: 321-376.

Hainsworth, P.J. and Garson, O.M. (1990). Breast cancer cytogenetics and beyond. Aust. N. Z. Surg. 60: 327-336.

Hampton, G.M., Mannermaa, A., Winquist, R., Alavaikko, M., Blanco, G., Taskinen, P.J., Kiviniemi, H., Newsham, I., Cavenee, W.K. and Evans, G.A. (1994). Loss of heterozygosity in sporadic human breast carcinoma: a common region between 11q22 and 11q23.3. Cancer Res. 54: 4586-4589.

HGM 11: Human Gene Mapping 11 (1991). Cytogenet. Cell Genet. 58.

Hill, M.S., Rodgers, C.S. and Hultén, M.A. (1987). Cytogenetic analysis in human breast carcinoma. II. Seven cases in the triploid/tetraploid range investigated using direct preparations. Cancer Genet. Cytogenet. 24: 45-62.

ISCN (1991). Guidelines for cancer cytogenetics. Supplement to: An International System for Human Cytogenetic Nomenclature (Mitelman, F., ed.). S. Karger, Basel, pp. 56.

Leonessa, F., Boulay, V., Wright, A., Thompson, E.W. and Clarke, R. (1992). The biology of breast tumor progression. Acta Oncol. 31: 115-123.

Li, F.P. (1993). Molecular epidemiology studies of cancer in families. Br. J. Cancer 68: 217-219.

Lópes-Guinés, C., Callahan, R.C., Ruiz, A., Gilrpellin, A., Calderón, J., Vasquez, C. and Llonbartbosch, A. (1991). Cytogenetics, flow cytometry, cytophotometry of 22 cases of primary breast carcinoma. A comparative study. Virchows Archiv. B Cell Pathol. 61: 133-140.

Mitelman, F. (1991). Catalog of Chromosome Aberrations in Cancer. Willey & Sons, New York, pp. 1987.

Negrini, M., Rasio, D., Hampton, G.M., Sabbioni, S., Rattan, S., Carter, S.L., Rosenberg, A.L., Schwartz, G.F., Shiloh, Y., Cavenee, W.K. and Croce, C.M. (1995). Definition and refinement of chromosome 11 regions of loss of heterozygosity in breast cancer: identification of a new region at 11823.3. Cancer Res. 55: 3003-3007.

Pathak, S., Wang, Z., Dahallwal, M.K. and Sacks, P.C. (1988). Telomeric association: another characteristic of cancer chromosomes? Cytogenet. Cell Genet. 47: 227-229.

Ponder, B. (1988). Gene losses in human tumors. Nature 335: 400-402.

Rodgers, C.S., Hill, S.M. and Húlten, M.A. (1984). Cytogenetic analysis in human breast carcinoma. I. Nine cases in the diploid range investigated using direct preparations. Cancer Genet. Cytogenet. 18: 95-119.

Sager, R. (1989). Tumor suppressor genes. The puzzle and the promise. Science 246: 1406-1412.

Sandberg, A.A. (1990). The chromosome in human cancer and leukemias. Hematol. 23: 201-217.

Seizinger, B.R., Klinger, H.P., Junien, C., Nakamura, Y., Le Beau, M., Cavenee, W., Emanuel, B., Ponder, B., Naylor, S., Mitelman, F., Louis, D., Menon, A., Newsham, I., Deker, J., Koelbling, M., Henry, I. and Deimling, A.V. (1991). Report of the committee on chromosome and gene loss in human neoplasia. Cytogenet. Cell Genet. 58: 1080-1096.

Thompson, A.M., Moris, R.G., Wallace, M., Wyllie, A.H., Steel, C.M. and Carter, D.C. (1993). Allele loss from 5q21 (APC/MCC) and 18q21 (DCC) and mRNA expression in breast cancer. Br. J. Cancer 68: 64-68.

Van de Vijver, M.J. (1993). Molecular genetic changes in human breast cancer. Adv. Cancer Res. 61: 25-55.

Vogelstein, B., Fearon, E.R., Stanley, B.A., Hamilton, R., Kern, S.E., Preisinger, A.C., Leppert, M., Nakamura, Y., White, R., Smits, A.M.M. and Bos, J.L. (1988). Genetic alterations during colorectal tumor development. N. Engl. J. Med. 319: 525-532.

Weinberg, R.A. (1991). Tumor suppressor genes. Science 254: 1138-1145.

Weinstein, B. (1991). Mitogenesis is only one factor in carcinogenesis. Science 251: 387-388.

Wolman, S.R. (1986). Cytogenetic heterogeneity: Its role in tumor evolution. Cancer Genet. Cytogenet. 19: 129-140.

Yunis, J.J. (1981). New chromosome techniques in the study of human neoplasia. Hum. Pathol. 12: 540-549.