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Identification of the K-casein and b-lactoglobulin genotypes in Brazilian Bos indicus and Bubalus bubalis populations

Sílvia Nassif Del Lama^I; Marco Antônio Zago^II

^IDepartamento de Genética e Evolução, Universidade Federal de São Carlos, 13565-905 São Carlos, SP, Brasil. Send correspondence to S.N.D.L.
^IIDepartamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brasil

ABSTRACT

Blood samples from two Bos indicus breeds, Nelore and Gyr, and from one water buffalo herd, Bubalus bubalis, raised in Brazil, were genotyped for kappa-casein and beta-lactoglobulin loci. Variants A and B were identified at two loci by polymerase chain reaction and digestion with Hind III and Hph I. The lengths of the amplified and digested fragments from cattle and buffalo DNA were similar. Genetic variation was detected in the bovine breeds but no polymorphism was observed in the buffalo population, which presented k-Cn B and b-Lg B alleles. The frequencies of B alleles at the k-casein and b-lactoglobulin loci were 0.08 and 0.77 in Nelore, and 0.06 and 0.62 in Gyr. Compared to the original Indian breeds, the Brazilian bovine populations showed a significant decrease in the frequency of the k-Cn B allele and non-significant differences in the frequency of b-Lg B. Genotyping of males and young animals is recommended for the planning of matings to increase the k-Cn B allele frequency in Brazilian populations.

Keywords: K-casein; b-lactoglobulin; Bos indicus; Bubalus bubalis.

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