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A simple and fast procedure to grow bat fibroblasts from lung biopsies for cytogenetic studies

Eliana Morielle-Versute^I; Marileila Varella-Garcia^II

^IDepartamento de Zoologia, Instituto de Biociências, Letras e Ciências Exatas, UNESP, Caixa Postal 136, 15054-000 São José do Rio Preto, SP, Brasil. Send correspondence to E.M.-V.
^IICancer Center Cytogenetics Core, University of Colorado Health Sciences Center, Denver, CO, USA

ABSTRACT

A fast, simple, and inexpensive procedure to establish fibroblast culture from bat lungs is presented. Explants plated following mechanical disaggregation provide good quality preparations for cytogenetics studies in about one week. Cultures established with this procedure may also be used for other biological studies.

Keywords: bat; fibroblasts; lung biopsies; cytogenetic.

REFERENCES

Baker, R.J. and Haiduk, M. (1985). Collections of tissue culture cell lines suspended by freezing. Acta Zool. Fennica 170: 91-92.

Morielle-Versute, E. (1992). Estudo citogenético em espécies da família Molossidae (Chiroptera, Mammalia). Doctoral Thesis, Instituto de Biociências, Letras e Ciências Exatas, UNESP, São José do Rio Preto, SP.