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A simple technique for isolation of DNA suitable for PCR amplification from cytogenetic preparations

Mônica Vannucci Nunes^I; Mônica Barbosa de Melo^II; Fernando Ferreira Costa^II; Marileila Varella-Garcia^I

^IIBILCE, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto, Departamento de Biologia, UNESP, Caixa Postal 136, 15054-000 São José do Rio Preto, SP, Brasil. Send correspondence to M.V.N.
^IIHemocentro, UNICAMP, Campinas, SP, Brasil

ABSTRACT

In order to rescue molecular information from chromosome preparations, we describe a rapid procedure to obtain DNA from cytogenetic preparations in microscope slides, stored for one to five years at room temperature. This technique was modified from previously described procedures and the DNA obtained was shown to be suitable for PCR amplification.

Keywords: DNA suitable; PCR amplification; cytogenetic.

REFERENCES

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Koremberg, J. and Freedlander, E. (1974). Giemsa technique for the detection of sister chromatid exchanges. Chromosome 48: 335-360.

Melo, M.B., Sales, T.S.I., Lorand-Metze, I. and Costa, F.F. (1992). Rapid method for isolation of DNA from glass slide smears for PCR. Acta Haematologice 87: 214-215.