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Mechanisms of 2n potato pollen formation in dihaploid Solanum tuberosum L. x S. chacoense Bitt. hybrid clones^*

Marcelo Nascimento de Oliveira; Lisete Chamma Davide; César Augusto Brasil Pereira Pinto

Departamento de Biologia, Universidade Federal de Lavras, Caixa Postal 37, 37200-000 Lavras, MG, Brasil. Send correspondence to L.C.D.

ABSTRACT

The backcrosses of dihaploid Solanum tuberosum with wild species hybrids generating tetraploids progenies require the formation of non-reduced pollen. In this work, the mechanisms responsible for the formation of 2n pollen in 28 dihaploid Solanum tuberosum x Solanum chacoense hybrids were sredied. Four mechanisms were found: parallel spindles (ps), fused spindles (fs), premarere cytokinesis-1 (pc-1) and premarere cytokinesis-2 (pc-2). The ps mechanism was the most frequent, being found in 23 of the 28 assessed clones. The ps and fs mechanisms led to the formation of dyads by first division restiretion (FDR), transferring about 80% of the heterozygosity to the progenies. The pc-1 and pc-2 mechanisms also led to the formation of dyads, but they were genetically equivalent to second division restiretion (SDR), transferring only 40% of the heterozygosity to the progenies. Occurrence of FDR and SDR were shown to be associated in 12 clones, indicating that the clones can produce non-reduced microspores by more than one mechanism. However, only one mechanism is functional in a single pollen-grain mother-cell. Clones 9-2, 9-3, 9-6 and 15-15 are recommended for use in 4x x 2x matings.

Keywords: potato pollen; dihaploid; Solanum tuberosumL.; S. chacoense Bitt.

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* Part of a thesis presented by M.N.O. to Universidade Federal de Lavras (UFLA) in partial fulfillment of the requirements for the Master's degree.