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A study of biochemical markers of Kale (Brassica oleracea var. acephala) clones resistant to aphids (Brevicoryne brassicae)

M.A.N. Sediyama^I; A.T. Amaral Júnior^II; D.J.H. da Silva^II; M.C. Picanço^III; C.D. Cruz^IV

^IEPAMIG, Empresa de Pesquisa Agropecuária de Minas Gerais, Caixa Postal 216, 36570-000 Viçosa, MG, Brasil. Send correspondence to M.A.N.S.
^IIDepartamento de Fitotecnia, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil
^IIIDepartamento de Biologia Animal, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brasil
^IVDepartamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil

ABSTRACT

Seven kale clones (Brassica oleracea var. acephala), assessed for resistance to aphids (Brevicoryne brassicae) by Paula et al. (An. Soc. Entomol. Bras. 24: 99-104, 1995) underwent isozyme analysis using Esterase (EST), Acid Phosphatase (ACP), Glutamate dehydrogenase (GDH), Glutamate Oxaloacetate Transaminase (GOT), Leucine Aminopeptidase (LAP), Malate Dehydrogenase (MDH) and Peroxidase (PO) systems to identify resistant genotypes. EST, the only monomorphic enzyme was inefficient for discriminating between genotypes, whereas ACP, because of its accentuated polymorphisms, allowed the separation of five out of seven of the clones. No association between the ACP loci and resistance or susceptibility to plant aphid was established. However, ACP using standard 5, and GOT using standard 9, identified the Venus clone, one of the most resistant to the pest. The other systems were not efficient in separating clones or in identifying resistance, because of the coincidence of the isozyme patterns.

Keywords: biochemical markers; Kale; clones; aphids.

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