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Susceptibility of some Brazilian soybean genotypes to three strains of Agrobacterium tumefaciens

A. O. Mauro^I; J. C. Nóbrega^I; G. L. Baraldi^I; G. B. Collins^II

^IDepartamento de Fitotecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade do Estado de São Paulo, Campus de Jaboticabal, 14870-000 Jaboticabal, SP, Brasil. Send correspondence to A.O.M.
^IIDepartment of Agronomy, University of Kentucky, Lexington, KY 40546-0091, USA

ABSTRACT

Twenty-six soybean (Glycine max (L.) Merrill) genotypes were screened for susceptibility to three strains of Agrobacterium tumefaciens. The genotype Peking, the most susceptible cultivar known, was used as the control. The strains of A. tumefaciens used included: C-58 (nopaline), Bo-542 (agropine), and Ach-5 (octopine). The cultivars were evaluated in the greenhouse in a randomized complete block design, with two replications. The genotypes IAC-5, IAC-14, and Peking were the most susceptible to strain C-58; IAC-4, IAC-10, and Peking were susceptible to strain Ach-5; and IAC-5, IAC-7 and IAC-12 were the most susceptible to strain Bo-542, with susceptibility greater than for Peking. Strong and significant interaction between genotypes and strains of A. tumefaciens was also observed.

Keywords: soybean genotypes; Agrobacterium tumefaciens.

REFERENCES

Bailey, M.A., Boerma, H.R. and Parrott, W.A. (1994). Inheritance of Agrobacterium tumefaciens-induced tumorogenesis of soybean. Crop Sci. 34: 514-519.

Byrne, M.C., McDonnell, R.E. and Wright, M.S. (1987). Strain and cultivar specificity in the Agrobacterium-soybean interaction. Plant Cell Tissue Organ Cult. 8: 3-15.

De Cleene, M. and De Ley, J. (1976). The host range of crown gall. Bot. Rev. 42: 389-466.

Delzer, B.W., Somers, D.A. and Orf, J.H. (1990). Agrobacterium tumefaciens susceptibility and plant regeneration of 10 soybean genotypes in maturity groups 00 to II. Crop Sci. 30: 320-322.

Fehr, W.R. and Caviness, C.E. (1977). Stages of soybean development Spec. Rep. n. 80. Coop. Ext. Serv., Agric. and Home Economics. Exp. Stn., Iowa State University Press. pp. 32.

Hood, E.E., Jen, G., Kayer, L., Kramer, J., Fraley, R.I. and Chilton, M.D. (1984). Restriction endonuclease map of pTiBo542, a potential Ti plasmid vector for genetic engineering of plants. Biotechnology 2: 702-709.

Matthysse, A.G. and Gurlitz, R.H.G. (1982). Plant cell range for attachment of Agrobacterium tumefaciens to tissue culture cells. Phys. Plant. Path. 21: 381-387.

Mauro, A.O., Pfeiffer, T. and Collins, G.B. (1992). Genetics of gall formation and relationship between gall formation and transformability in soybean. In: Crown Gall Meetings. Raleigh, North Carolina. Abstracts. pp. 6.

Mauro, A.O., Pfeiffer, T. and Collins, G.B. (1994). Conhecimento básico para estudos que envolvam inoculações com Agrobacterium tumefaciens em soja. Cientifica 22: 187-194.

Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-477.

Owens, L.D. and Cress, D.E. (1985). Phenotypic variability of soybean response to Agrobacterium strains harbouring the Ti or Ri plasmids. Plant Physiol. 77: 87-94.

Pendersen, H.C., Christiansen, J. and Wyndaele, R. (1983). Induction and in vitro culture of soybean crown gall tumors. Plant Cell Rep. 2: 201-204.

Snedecor, G.W. and Cochran, W.G. (1989). Statistical Methods. 8th edn. Iowa State University Press, Ames, pp. 503.

Uchimiya, H., Handa, T. and Brar, D.S. (1989). Transgenic plants. J. Biotechnol. 12: 1-20.

van Wordragen, M.F. and Dons, H.J.M. (1992). Agrobacterium tumefaciens mediated transformation of recalcitrant crops. Plant Mol. Biol. Rep. 10: 12-38.

Weising, K., Schell, J. and Kahl, G. (1988). Foreign genes in plants: Transfer, structure, expression and applications. Annu. Rev. Genet. 22: 421-477.

Wyndaele, R., Van Onckelen, H., Christiansen, J., Rudelshein, P., Hernans, R. and De Greef, J. (1985). Dynamics of endogenous IAA and cytokinins during the drowth cycle of soybean crown gall and untransformed callus. Plant Cell Physiol. 22: 1147-1154.