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Restriction endonuclease analysis of mitochondrial DNA of Metarhizium anisopliae strains

Haroldo Alves Pereira Júnior; Nilce M. Martinez-Rossi

Departamento de Genética, FMRP-USP, Av. Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brasil. Phone: (016) 633-3035 Ext. 390, Fax: (016) 633-0069, E-mail: NMMROSSI@fmrp.usp.br. Send correspondence to N.M.M.-R.

ABSTRACT

Mitochondrial DNAs from strains A4, A19, AL, E6, E9 and MT of Metarhizium anisopliae var. anisopliae were isolated and characterized by restriction-endonucleases. The banding pattern obtained was the same for all strains investigated, indicating that their mtDNAs have the same molecular sizes (about 36 Kb) and the same restriction sites for the enzymes used. Thus, the restriction pattern of the mtDNA of these strains may serve as a specific marker for the identification of the variety anisopliae or of subpopulations of this variety.

Keywords: endonuclease; mitochondrial; Metarhizium anisopliae.

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