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A DNA probe to identify enterotoxigenic Escherichia coli expressing PCF0159 colonization factor

Wanderley Dias da Silveira^I; Fabiana Fantinatti^I; Ben Tall^II; James Kaper^III; Carol Tacket^III

^IDepartamento de Microbiologia e Imunologia, Universidade Estadual de Campinas, 13081-970 Campinas, SP, Brasil. Send correspondence to W.D.S.
^IIFood and Drug Administration, Washington, DC, USA
^IIICenter for Vaccine Development, University of Maryland, Baltimore, MD, USA

ABSTRACT

The expression of PCF0159 fimbriae by a ETEC Escherichia col strain was blocked by using transposon TnphoA. The plasmidial DNA sequence which includes the insertion of the transposon was cloned and used as a DNA probe against E. coli strains expressing different colonization factors and enterotoxins. The DNA probe was specific only for those strains belonging either to the serotype 0159:H4 or carrier strains which expressed PCF0159 fimbriae operons.

Keywords: DNA probe; enterotoxigenic; Escherichia coli.

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