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Determination of genetic diversity within Brazilian soybean germplasm using random amplified polymorphic DNA techniques and comparative analysis with pedigree data

Ricardo Vilela Abdelnoor; Everaldo Gonçalves de Barros; Maurílio Alves Moreira

Núcleo de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil. Send conespondence to M.A.M.

ABSTRACT

We used the random amplified polymorphic DNA (RAPD) technique to evaluate genetic diversity among 38 Brazilian soybean (Glycine max L. Merrill) cultivars. The analysis allowed the separation of the cultivars into five distinct groups based on the genetic distances among them. Comparison between these results and pedigree data were coherent for most cases. The data confirmed the use of the RAPD technique as a potent tool to aid the study of genetic diversity among individuals of the same species and should allow Brazilian soybean breeders a more rational choice of progenitors to be used in specific breeding programs.

Keywords: genetic diversity; Brazilian soybean; germplasm; polymorphic DNA; pedigree data.

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