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Identification of a periplasmic protein associated with osmolarity-dependent aminoglycoside resistance in Escherichia coli

Mônica B. Rodriguez^I; Luis Carlos S. Ferreira^II; Gisele Monteiro^I; Sérgio Olavo P. da Costa^I

^IUniversidade de São Paulo, Laboratório de Genética de Microrganismos, ICB II, Av. Prof. Lineu Prestes 1374, 05508-900 São Paulo, SP, Brasil Fax: 005511 8130845. Send correspondence to S.O.P.C.
^IIInstituto de Biofísica Carlos Chagas Filho, UFRJ, Cidade Universitária, 21949-900 Rio de Janeiro, RJ, Brasil

ABSTRACT

A new type of osmolarity-dependent aminoglycoside resistant Escherichia coif mutant was isolated. Analysis of cell envelope proteins of one representative clone revealed a drastic reduction of a periplasmic protein with a relative molecular weight of 61 kDa. The involvement of this periplasmic protein in the uptake of aminoglycoside antibiotics and its possible identity with a transport protein of an oligopeptide uptake system is discussed.

Keywords: periplasmic protein; osmolarity-dependent; aminoglycoside; Escherichia coli.

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