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Cloning shrimp Baculovirus penaei DNA and hybridization comparison with Autographa californica nuclear polyhedrosis virus

Carlos R. Machado^I; Sérgio L. de S. Bueno^II; Carlos F.M. Menck^I

^IDepartamento de Biologia, Instituto de Biociências, Universidade de São Paulo, Caixa Postal 11461, 05422-970 São Paulo, SP, Brasil. Tel.: (011) 818-7587, Fax: (011) 818-7416. Send correspondence to C.F.M.M.
^IIDepartamento de Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil

ABSTRACT

A significant degree of specific homology between Baculovirus penaei (BP) - from infected shrimp feces and hepatopancreas - and Autographa califomica multiple nuclear polyhedrosis virus (AcMNPV) of insects was observed by dot blot hybridization. A genomic library of BP was constructed and some clones showed partial homology with AcMNPV DNA. Two of these clones were further characterized and one of them hybridized specifically with baculovirus DNA. This baculovirus DNA may be employed as a probe for detecting BP infected shrimp.

Keywords: Baculovirus penaei; DNA; hybridization; Autographa californica; nuclear polyhedrosis; virus.

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