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A simplified method for the isolation of high molecular weight DNA from Aspergillus nidulans

 

 

Cristina Andrade-MonteiroI; Walter Maccheroni JuniorI; Antonio RossiII; Nilce M. Martinez-RossiI

IDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, USP, 14049-900 Ribeirão Preto, SP, Brasil. Send correspondence to N.M.M.-R.
IIDepartamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, USP, 14040-901 Ribeirão Preto, SP, Brasil

 

 

ABSTRACT

We have developed a simple method for obtaining DNA from mycelium of the fungus Aspergillus nidulans. A single isopropanol preparation yields good quality high molecular weight DNA preparations that are not contaminated with proteins or salts, and that are easy to solubilize and to digest with restriction enzymes. High yields (approximately 1.6 mg DNA per gram of wet mycelium) are obtained. Contamination with RNA is minimal and there is no need to use RNase. It has been successfully used in our laboratory for many molecular biology experiments.

Keywords: isolation; high molecular weight; DNA; Aspergillus nidulans.

 

 

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