Full text in pdf format

Polymorphism of trehalose accumulation in sibling species of Saccharomyces sensu stricto

Elena Naumova^I; Gennadi Naumov^I; Anita D. Panek^II

^IState Scientific-Research Institute for Genetics and Selection of Industrial Microorganisms, Dorozhnyi 1, Moscow 113545, Russia
^IIDepartamento de Bioquímica, CT Bloco A, Lab 547, Instituto de Química, Universidade Federal do Rio de Janeiro, Caixa Postal 68653, 21941-009 Rio de Janeiro, RJ, Brasil. Fax (5521) 290-7248. Send correspondence to A.D.P.

ABSTRACT

Trehalose accumulation (Tac⁺) was studied in three sibling species Saccharomyces cerevisiae, S. paradoxus and S. bayanus. In the former two species, a new type of Tac⁺ was found in non-maltose fermenting strains, which was not controlled by known maltose regulatory genes. Strains with active Tac⁺ patterns were selected. In strain N25 of S. paradoxus the Tac⁺ property is controlled by two complementary genes while in S. cerevisiae, strain UFRJ 50613, several polymeric cumulative genes are probably responsible for the high level of trehalose accumulation. This new Tac⁺ phenotype apparently is characteristic of wild strains of Saccharomyces s.str.

Keywords: polymorphism; trehalose; sibling species; Saccharomyces.

REFERENCES

Boucherie, H. (1985). Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae. J. BacterioL 161: 385-392.

Brin, M. (1966). Transketolase: clinical aspects. In: Methods in Enzymology (Colowick, S.P. and Kaplan, N.O., eds.), vol. 9. Academic Press, New York, pp. 506-514.

Charron, M.J., Read, E., Haut, S.R. and Michels, C.A. (1989). Molecular evolution of the telomere-associated MAL loci of Saccharomyces. Genetics 122: 307-331.

Naumov, G.I. (1977). Comparative genetics of yeasts. XVI. Genes for maltose fermentation in Saccharomyces carlsbergensis N.C.Y.C.74. Sov. Genet. 12: 1374-1385.

Naumov, G.I. (1987). Genetic basis for classification and identification of the ascomycetous yeasts. Studies in Mycology 30: 469-475.

Naumov, G.I. and Gudkova, N.K. (1979). Comparative genetics of yeast XVIII. Microevolution of Saccharomyces bayanus. Soy. Genet. 15: 380-387.

Naumov, G.I. and Naumova, E.S. (1991). A wild yeast population of Saccharomyces cerevisiae found in Siberia. Microbiologia 60: 537-540 (in Russian).

Naumov, G.I. and Nikonenko, T.A. (1988). The East Asia is a probable land of the cultured yeasts of Saccharomyces cerevisiae. Izv. Sib. otd. Akad. Nauk SSSR Ser. Biol. Nauk 20: 97-101 (in Russian).

Naumov, G.I. and Nikonenko, T.A. (1989). Occurrence and physiological characteristics of biological species Saccharomyces bayanus from hybridological analysis. Microbiology 57: 526-530.

Naumov, G.I., Kondratieva, V.I., Naumova, T.I. and Gudkova, N.K. (1983a). Genetic bases for classification of Saccharomyces cerevisiae. A study of survival of hybrid ascospores. Zh. Obs. Biol. 44: 648-660 (in Russian).

Naumov, G.I., Kondratieva, V.I., Naumova, T.I. and Gudkova, N.K. (1983b). Genetic inbred lines of yeasts Saccharomyces cerevisiae Hansen. Mikol. Fitopatol.17: 476-480 (in Russian).

Naumov, G.I., Kondrat'eva, V.I. and Naumova, E.S. (1986). Methods for hybridization of homothallic yeast diplonts and haplonts. Sov. Biotechnol. 6: 29-32.

Naumov, G.I., Naumova, E.S. and Michels, C.A. (1991). Identification of the functional a-glucosidase gene in natural mutants of Saccharomyces cerevisiae and S. paradoxus non-fermenting maltose. Dokl. Akad. Nauk SSSR 316: 1249-1252 (in Russian).

Naumov, G., Naumova, E. and Korhola, M. (1992a). Genetic identification of natural Saccharomyces sensu stricto yeasts from Finland, Holland and Slovakia. Antonie van Leeuwenhoek 61: 237-243.

Naumov, G.I., Naumova, E.S., Lantto, R.A., Louis, E.J. and Korhola, M. (1992b). Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and s. bayanus: electrophoretic karyotypes. Yeast 8: (in press).

Naumova, E.S., Naumov, G.I., Michels, C.A. and Beritashvili, D.R. (1991). Identification of chromosomal DNA patterns of the species Saccharomyces bayanus and S. pastorianus. Dokl. Akad Nauk SSSR 316: 744-746 (in Russian).

Needleman, R. (1991). Control of maltase synthesis in yeast. Molecular Microbiology 5: 2079-2084.

Oliveira, D.E., Rodrigues, E.G.C., Mattoon, J.R. and Panek, A.D. (1981). Relationships between trehalose metabolism and maltose utilization in Saccharomyces cerevisiae II. Effect of constitutive MAL genes. Current Genetics 3: 235-242.

Panek, A.D. and Panek, A.C. (1990). Metabolism and thermotolerance function of trehalose in Saccharomyces: a current perspective. J. Biotechnology 14: 229-238.

Panek, A.D., Sampaio, A.L., Braz, G.C., Baker, S.J. and Mattoon, J.R. (1980). Genetic and metabolic control of trehalose and glycogen synthesis. New relationships between energy reserves, catabolite repression and maltose utilization. Cellular and Molecular Biology 25: 345-354.

Paschoalin, V.M.F., Panek, A.C. and Panek, A.D. (1987). Catabolite inactivation of trehalose synthesis during growth of yeast on maltose. Brazilian J. Med. Biol. Res. 20: 675-683.

Trevelyan, W.E. and Harrison, J.S. (1952). Studies of yeast metabolism. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50: 298-302.

Vaughan Martini, A. (1989). Saccharomyces paradoxus comb. nov., a newly separated species of the Saccharomyces sensu stricto complex based upon nDNA/nDNA homologies. Syst. Appl. Microbiol.12: 179-182.