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Susceptibility of brazilian soybean cultivars to Agrobacterium tumefaciens

Annette Droste^I; Maria Helena Bodanese-Zanettini^I; Elsa Mundstock^II; Ching-Yeh Hu^III

^IDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-97Q Porto Alegre, RS, Brasil. Send correspondence to M.H.B.-Z.
^IIDepartamento de Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
^IIICenter for Applied Science, William Paterson College, Wayne, N.J., USA

ABSTRACT

Transformation of soybean (Glycine max (L.)Merr.) via Agrobacterium is affected by the susceptibility of genotypes to specific Agrobacterium strains. Four Brazilian soybean cultivars were screened withA. tumefaciens strains A281::pZA₃, A208 and C58. The tumor frequency and size varied significantly among cultivars and strains. The relative frequency of tumor formation was greatest for strains A208 and C58 on all soybean cultivars tested. The cultivar BRAGG was most susceptible. Tumors showed hormone-independent growth. Tumor segments were opine positive. Tumors induced by A281::pZA₃ also expressed the reporter GUS gene. The results indicated that some of the Brazilian soybean cultivars, e.g. BRAGG, can be used in Agrobacterium mediated gene transfer experiments.

Keywords: soybean; cultivars; Agrobacterium tumefaciens.

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