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Genetic recombination by protoplast fusion in the deuteromycete Beauveria bassiana

Luzia Doretto Paccola-Meirelles^I; João Lúcio Azevedo^II

^ICentro de Ciências Biológicas, Departamento de Biologia Geral, Universidade Estadual de Londrina, Caixa Postal 6001, 86051-970 Londrina, PR, Brasil. Send correspondence to L.D.P.M.
^IIEscola Superior de Agricultura "Luiz de Queiroz", Instituto de Genética, Universidade de Sao Paulo, Piracicaba, SP, Brasil

ABSTRACT

Protoplasts of auxotrophic mutants of Beauveria bassiana were produced by treating germ tubes with lytic enzymes. Regeneration frequency was 33%. The fusogenic agent used was polyethylene glycol and the fusion products were highly unstable. The sectors which emerged from the fusion products were analyzed and recombinants were obtained. The electrophoretic pattern for esterases of parental strains and recombinant sectors was determined. The protoplast fusion technique proved to be a valuable tool for studies of genetics and breeding of B. bassiana.

Keywords: genetic recombination; protoplast fusion; deuteromycete; Beauveria bassiana.

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