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The effects of 3-Aminobenzamide and Aphidicolin on X-ray-induced chromosomal aberrations in cycling and Non-cycling down lymphocytes

Awadhesh Nandan Jha^I,III; Tikaram Sharma^I; Golearan Prasad Katiyar^II

^ICytogenetics Laboratory, Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi-221 005, India
^IIDepartment of Pediatrics, Institute of Medical Science, Banaras Hindu University, Varanasi-221 005, India
^IIIPresent address: Brixham Environmental Laboratory, ZENECA Limited, Brixham, Devon TQ5 8BA, UK. Send correspondence to A.N.J.

ABSTRACT

Aphidicolin (APC) and 3-aminobenzamide (3AB) were used to elucidate the role of DNA repair processes in the increased production of X-ray-induced chromosomal aberrations in lymphocyte cultures from Down syndrome patients (Downs), compared to normal controls. In the phytohaemagglutinin (PHA)-stimulated condition (G1 phase) the normal controls showed a significant increase in the yield of dicentrics, but not acentrics. In the Down cultures, the stimulated (G1) compared to unstimulated (GO) cells showed a significant increase of dicentrics as well as acentrics. 3AB potentiated the yield of aberrations only in stimulated lymphocytes of the groups, more in normal controls than in Downs. The potentiating effect of APC, in contrast, was more pronounced in the unstimulated compared to stimulated condition. In the stimulated condition, like 3AB, the potentiating effect of APC was greater in normal controls. The differential processing of X-ray-induced genetic damage in peripheral lymphocytes from Downs is sensitive to the presence of APC and 3AB. It is likely that both DNA polymerase a and poly (ADP-ribose) polymerase have inherent differential behaviour in PHA-responsibe Down lymphocytes.

Keywords: 3-Aminobenzamide; aphidicolin; x-Ray-induced; chromosomal aberrations; cycling; non-cycling; lymphocytes.

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