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Evaluation of the genotoxic activity of the sesquiterpene lactone goyazensolide in mammalian systems in vitro and in vivo

Mário Sérgio Mantovani^I; Catarina Salle Takahashi^I,II; Walter Vichnewski^III

^IDepartamento de Genética, Faculdade de Medicina, USP, Avenida Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brasil. Send correspondence to M.S.M.
^IIDepartamento de Biologia, Faculdade de Filosofia Ciências e Letras, USP, 14049-900 Ribeirão Preto, SP, Brasil
^IIIDepartamento de Física e Química, Faculdade de Ciências Farmacêuticas, USP, 14049-900 Ribeirão Preto, SP, Brasil

ABSTRACT

The sesquiterpene lactone goyazensolide has schistosomicidal and cytotoxic activities. This chemical substance was tested to determine clastogenic effects on mammalian systems in vitro (human peripheral blood lymphocyte cultures) and in vivo (Wistar rat bone marrow cells). The concentrations tested in vitro were 0.1, 0.3, 0.6 and 1.0 mg/ml culture medium, and in vivo were 0.4, 0.8, 1.6 and 3.2 mg/ 100 g body weight. All in vitro treatments with goyazensolide and only the 0.8 mg in vivo treatment had significantly more chromosome gaps than the controls. However, only in vitro analysis of the number of cells with chromosome breaks demonstrated that treatment with 0.6 mg goyazensolide/ml culture medium has a clastogenic action. Goyazensolide was found to have no effect on SC induction in vitro. The toxic dose was determined both in vitro (1.0 mg/ml) and in vivo (3.2 mg/100 g).

Keywords: genotoxic activity; sesquiterpene lactone; goyazensolide; mammalian systems.

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