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Chromosomal aberrations induced with endonucleases^*

Günter Obe; Christian Johannes

Department of Genetics, University GH Essen, P.O. Box 45037, D-45117 Essen, Germany. Send correspondence to G.O.

ABSTRACT

Experiments with Neurospora crassa endonuclease, pancreatic DNase I and bacterial restriction endonucleases indicate that DNA double-strand breaks are ultimate lesions for the induction of chromosomal aberrations. Cellular uptake of such enzymes can be mediated by different methods which are discussed here.

Keywords: chromosomal aberrations; endonucleases.

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* This paper was presented at the ICRO/UNESCO International Training Course "Molecular Organization of the Eukaryotic Chromosome in Relation to the Induction of Chromosome Aberrations" organized by Professor Günter Obe, Dr. Máximo E. Drets and Dr. Gustavo A. Folle, held September 16-28, 1991 in Montevideo, Uruguay. It is the fourth in a series of five papers from this course which will be published in the Brazilian Journal of Genetics.