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Affinity grouping of closely related Lines of dry beans (Phaseolus Vulgaris L.) through comparative electrophoresis of seed proteins

Sérgio Echeverrigaray^I; Maria Teresa V. Carvalho^II; Antonio S. Pompeu^III; Eric Derbyshire^II

^IPresent address - Instituto de Biotecnologia, Universidade de Caxias do Sul, 95070 Caxias do Sul, RS, Brasil
^IISeção de Biologia de Proteínas Vegetais, Centro de Energia Nuclear na Agricultura, CENA/USP, 13418-900 Piracicaba, SP, Brasil. Send correspondence to E.D.
^IIISeção de Genética, Instituto Agronômico, 13001-970 Campinas, SP, Brasil

ABSTRACT

The variation in seed polypeptides and basic proteins revealed by electrophoretic profiles of 26 lines of Phaseolus vulgaris L. were statistically analyzed. Cluster and principal component analysis allowed for the construction of six affinity groups and these were consistent with the genealogical data. This relationship indicates the reliability of analysis by seed protein electrophoresis for the estimation of genetic relationships among common bean lines. The correlation observed between an isolectin band with a high contribution in the first principal component of the multivariate analysis and the capacity of extracts to agglutinate cow erythrocytes suggests the possible utility of simple agglutination tests as a discriminatory variable, even among related lines.

Keywords: affinity grouping; dry beans; electrophoresis; seed proteins.

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