Full text in pdf format

Oil Palm (Elaeis guineensis, JACQ.) esterase genetic polymorphism revealed by isoelectric focusing in polyacrylamide gel

Márcio de Miranda Santos^I; Aguinaldo Luiz Simões^II; Moacyr Antonior Mestrine^II

^ICentro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, EMBRAPA, Caixa Postal 02372, 70849-970 Brasília, DF, Brasil. Send correspondence to M.M.S.
^IIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brasil

ABSTRACT

Oil Palm pollen extracts of 97 palms from 31 open pollinated progenies from Bahia State, Brazil, were analyzed for fluorogenic esterases through polyacrylamide gel isoelectric focusing at two pH gradients. There were a large number of bands distributed over five activity regions. Regions A, B and C presented monomorphic patterns and isozymes of region D were difficult to analyze.
The observed profile for region E (esterases E1 and E2) suggests that the control of this esterase is determined by two alleles and that this enzyme has a monomeric structure. Allelic and genotypic frequencies are presented for this locus. A hypothesis for genetic control of the two isozyme variants detected is presented.

Keywords: Oil Palm; esterase; polymorphism; polyacrylamide gel.

REFERENCES

Coates, P.M., Mestriner, M.A. and Hopkinson, D.A. (1975). A preliminary genetic interpretation of esterase isozymes of human tissues. Ann. Hum. Genet. 30: 1-20.

Ghesquiére, M. (1983). Contribution of l'etude de la variabilité genetique du palmier a huile (Elaeis guineensis, Jacq.): Le polymorphism enzymatique.

Doctoral Thesis, Université de Paris Sud, Orsay. Harris, H. (1980). The Principles of Human Biochemical Genetics. Elsevier, Amsterdam.

Loening, E.E. (1967). The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. J. Biochem. 102: 251-275.

Tanksley, S.D. and Orton, T.J. (1983). Isozymes in Plant Genetics and Breeding - Part B. Elsevier, Amsterdam, pp. 472.