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Inter-laboratory calibration program for the mouse micronucleus test

Lucia Regina Ribeiro^I; Catarina Satie Takahashi^II; Bernardo Erdtman^III; Susie V. Oliveira^I; Caio Tales Alvares da Costa^I; Maria Clara Gimmeler-Luz^III

^ILaboratório de Genética Toxicológica, Escola de Medicina Veterinária, UFBA, Avenida Ademar de Barros, 500, Ondina, 40210 Salvador, BA, Brasil
^IIFaculdade de Filosofia Ciências e Letras de Ribeirão Preto, USP, 14040-901 Ribeirão Preto, SP, Brasil. Send correspondence to C.S.T.
^IIIDepartamento de Genética, UFRGS, 91505 Porto Alegre, RS, Brasil

ABSTRACT

The micronucleus test on mammalian bone marrow is widely accepted as a "screening" test for chromosome anomalies induced by clastogenic agents. The frequency of micronucleated polychromatic erythrocytes (MNPCEs) in the bone marrow of mice treated with cyclophosphamide (CP), bleomycin (BLEO) and benzidine (BZN) was determined in three different laboratories. Although the experiments were conducted independently, the results were quite similar for all three laboratories. The mean MNPCE frequencies induced by CP and BLEO were not statistically different. Only for the group treated with a single BZN dose were the mean frequencies statistically different between laboratories two and three. The homogeneous results obtained by the three laboratories for the micronucleus test indicate the efficacy of the method and its applicability for the identification of clastogenic agents.

Keywords: Calibration program; Micronucleus test.

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