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Quantification of protein variation in two natural. Populations of Spodoptera frugiperda (Lepidoptera: Noctumae)

Leda Maria Koelblinger Sodré de Lima^I; Eucleia Primo Betioli Contel^II; Pedro Hernan Cabello^III

^IDepartamento de Biologia Geral, Universidade Estadual de Londrina, Caixa Postal 6001, 86055-900 Londrina, PR, Brasil. Send correspondence to L.M.K.S.L.
^IIDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, 14049-900 Ribeirão Preto, SP, Brasil
^IIIDepartamento de Genética, Instituto Oswaldo Cruz, 21040 Rio de Janeiro, RJ, Brasil

ABSTRACT

We analyzed 22 loci of Spodoptera frugiperda from a previous electrophoretic study of 12 proteins (Brazil. J. Genetics. 13: 711-729, 1990) to estimate the degree of genetic variation within and between two populations separated by a distance of approximately 500 km. Eleven polymorphic loci were detected with a mean P value of 41.7%. The genotypic frequencies of the different loci were homogeneously distributed both between sexes (within each sample) and between samples. We estimated mean ± standard deviations (SD) of observed heterozygosity (Ho) to be 0.0816 ± 0.0002 and mean ± SD of expected heterozigosity (He) to be 0.0935 ± 0.0030. A comparative analysis of P and He showed that our data are quite similar to those obtained for other S. frugiperda populations. Joint analysis of the present data and the literature permitted us to estimate the P and He of S. frugiperda to be 47.2% and 0.0778 ± 0.1503, respectively, based on 36 loci.

Keywords: Protein variation; Spodoptera frugiperda; Lepidoptera.

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