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In situ mapping of the hsp70 locus in seven species of the willistoni group of Drosophila

Cristina B.C. Bonorino^I; Mônica Pereira^II; Carlos E.V. Alonso^II; Vera L.S. Valente^I; Eliana Abdelhay^II

^IDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-970 Porto Alegre, RS, Brasil. Fax (051) 336-2011. Send correspondence to V.L.S.V.
^IIInstituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21949 Rio de Janeiro, RJ, Brasil

ABSTRACT

The hsp70 locus was mapped by in situ hybridization of a biotinylated probe (pPW 229) to the polytene chromosomes of seven species of the willistoni group of Drosophila. In all of them, the probe hybridized mainly at a single site of the third chromosome, corresponding in each case to a heat-induced puff site. Southern blot analysis comparing the Eco RI digested DNA of the willistoni species with D. melanogaster revealed that a large segment of DNA homologous to the hsp70 probe detected in D. melanogaster does not exist in the willistoni group. These results suggest that this locus is not duplicated in the willistoni group as it is in the melanogaster group.

Keywords: Hsp70 locus; Willistoni group; Drosophila.

REFERENCES

Beverley, S.M. and Wilson, A.C. (1984). Molecular evolution in Drosophila and the higher Diptera. II. A time scale for fly evolution. J. Mol. Evol. 21: 1-13.

Bonorino, C.B.C., Silva, T.C., Abdelhay, E. and Valente, V.L.S. (1993). Heat shock genes in the willistoni group of Drosophila: induced puffs and proteins. Cytobios 73: 49-64.

Dobzhansky, T. (1950). The chromosomes of Drosophila willistoni. J. Hered. 41: 156-158.

Engels, W.R., Preston, C.R., Thompson, P. and Eggleston, N.B. (1986). In situ hybridization to Drosophila salivary chromosomes with biotynilated DNA probes and alkaline phosphatase. Focus 8: 6-8.

Evgen'ev, M.B., Kolchinsky, A., Levin, A., Preobrazhenskaya, O. and Sarkisova, E. (1978). Heat shock DNA homology in distantly related species of Drosophila. Chromosoma 68: 357-365.

Jowett, T. (1986). Preparation of nucleic acids. In: Drosophila: a practical approach (Roberts, D.B., ed.). IRL Press, Washington, D.C.

Kastritsis, C.D. (1966). A comparative chromosome study in the incipient species of the Drosophila paulistorum complex. Chromosoma 19: 208-222.

Lakovaara, S. and Saura, A. (1972). Location of Enzyme loci in chromosomes of Drosophila willistoni. Experientia 28: 355-356.

Leigh-Brown, A.J. and Ish-Horowicz, D. (1981). Evolution of the 87A and 87C heat shock loci in Drosophila. Nature 290: 677-682.

Lindquist, S. (1986). The heat shock response. Ann. Rev. Biochem. 55: 1151-1191.

Lindquist, S. and Craig, E. (1988). The heat shock proteins. Ann. Rev. Genet. 22: 631-677.

Livak, K.J., Freund, R., Schweber, M., Wensink, P.C. and Meselson, M. (1978). Sequence organization and transcription at two heat shock loci in Drosophila. Proc. Natl. Acad. Sci. (USA) 11: 5613-5617.

Marques, E.A., Napp, M., Winge, H. and Cordeiro, A.R. (1966). A cornmeal, soybean flour, wheat germ medium for Drosophila. Drosophila Inf. Serv. 41: 187.

Molto, M.D., Martinez-Sebastian, M.J. and de Frutos, R. (1991). Localization of the hsp70 gene in Drosophila guanche. Drosophila Inf. Serv. 70: 150-151.

Müller, H.J. (1940). Bearings of the Drosophila work on systematics. In: The New Systematics (Huxley, J., ed.). Clarendon Press, Oxford.

Pavan, C. (1946). Chromosomal variation in Drosophila nebulosa. Genetics 31: 546-557.

Pierce, D.A. and Lucchesi, J.C. (1980). Dosage compensation of X-linked heat shock puffs in Drosophila pseudoobscura. Chromosoma 76: 245-254.

Southern, E.M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517.

Valente, V.L.S. and Morales, N.B. (1982). Photomap and reference map of the salivary gland chromosomes of Drosophila cubana Townsend. Drosophila Inf. Ser^y 58: 146-148.