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Analysis of the restriction fragment of Eucalyptus urophylla, E. grandis and. E. citriodora

J.L.C. Wolff^I; S.W. McDowell^II; M.A. Machado^III

^IUniversity of Victoria, Department of Biology, Vitoria, B.C., Canada
^IICENARGEN, Embrapa, 70001 Brasilia, DF, Brasil
^IIIMS Plantas, Desenvolvimento e Produção, Caixa Postal 6616, 13082 Campinas, SP, Brasil. Send correspondence to M.A.M.

ABSTRACT

Restriction fragment length polymorphysm analysis was performed to assess the level of genetic variability three eucalypt species (Eucalyptus citriodora, E. grandis and E. urophylla) and to select genetic markers for the establishment of a linkage map. Homologous and heterologous probes were hybridized to total DNA, digested with the restriction enzymes Eco RI and Hind IlI. Very high levels of intra and inter-specific polymorphism were detected.

Keywords: Restriction fragment length; Eucalyptus urophylla; E. grandis; E. citriodora.

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