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Biology of amazonian anophelines. XIX. a-glycerophosphate dehydrogenase in Anopheles nuneztovari: (Diptera: Culicidae) ontogeny and genetic variatio

Vera Margarete Scarpassa^I; Wanderli Pedro Tadei^I,II

^ICoordenação de Pesquisa em Ciências da Sande, INPA, Caixa Postal 478, 69083-000 Manaus, AM, Brasil. Send correspondence to V.M.S.
^IIUNESP, São José do Rio Preto, SP, Brasil

ABSTRACT

During the ontogenetic development of Anopheles nuneztovari, the electrophoretic profiles of a-glycerophosphate dehydrogenase permitted the diagnosis of an activity region, near the origin, wliich increased in intensity towards the adult stage. From the first instar larvae to the 4-hour-old pupae there is low enzymatic activity. Intense activity is seen in 12-hour-old pupae and continues to the recently emerged adult. The profile of the larval stage sliowed a sliglit difference in migration compared to the pupal and adult profiles. This allowed us to raise the hypothesis of the existence of two forms of the enzyme, products of independent loci, or, amore probably and as has been widely demonstrated in the genus Drosophila, the product of one locus, but with post-synthesis modification based on a polypeptide.The more intense activity in the aduh stage may indicate that the enzyme presents greater concentrations in the thorax, having functions related to flight activity. Also cor^related to flight activity was the low level of allele variation.

Keywords: a-glycerophosphate dehydrogenase; Anopheles nuneztovari Diptera.

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