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Action of an extract of Stryphnodendron obovatum benth seed on rat bone marrow and on human lymphocytes

 

 

Veronica Elisa Pimenta Vicentini-DiasI,III; Catarina Satie TakahashiI,II

IDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto-USP, 14049-900 Ribeirão Preto, SP, Brasil
IIDepartamento de Biologia, Faculdade de filosofia, Ciências e Letras de Ribeirão Preto - USP, 14049-901 Ribeirão Preto, SP, Brasil
IIIDepartamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Av. Colombo, 3690, 87020-900 Maringá, PR, Brasil. Send correspondence to V.E.P.V.-D.

 

 

ABSTRACT

Strypnodendron obovatum Benth (Barbatimão) is an important source of tannin used in industry. This plant has different biological activities, including inhibition of embryo development in rats and toxicity to cattle when ingested, and is also used to treat some human diseases. Because of its wide distribution and use, we studied the effect of an extract of Barbatimão seeds on Wistar rat bone marrow cells (in vivo) and on human peripheral blood lymphocytes (in vitro) in terms of mutagenesis and induction of colchicine-metaphases. The treatments did not cause a statistically significant increase in the number of chromosome aberrations or SCEs in either test system used. In the tests on rats there were a small induction of colchicine-metaphases, and in the lymphocyte cultures, this induction was dependent on time and form of treatment. A cytotoxic effect occurred in the lymphocyte cultures, which was dependent on increasing concentrations and time of exposure to the extract. Some treated metaphases presented banded chromosomes.

Keywords: Stryphnodendron obovatum; Rat bone marrow; Human lymphocytes.

 

 

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